Accurate modeling of single-particle cryo-EM images quantitates the benefits expected from using Zernike phase contrast
نویسندگان
چکیده
منابع مشابه
Denoising and Covariance Estimation of Single Particle Cryo-EM Images
The problem of image restoration in cryo-EM entails correcting for the effects of the Contrast Transfer Function (CTF) and noise. Popular methods for image restoration include 'phase flipping', which corrects only for the Fourier phases but not amplitudes, and Wiener filtering, which requires the spectral signal to noise ratio. We propose a new image restoration method which we call 'Covariance...
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We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required precision. With this approach we obtained a 3.2 Å resolution reconstruction of the Thermoplasma acido...
متن کاملZernike Phase Contrast Cryo-Electron Tomography of Bacteria and Viruses
Cryo-electron tomography (cryo-ET) is currently the only method available through which pleomorphic biological specimens are imaged to produce three-dimensional (3D) structures at ~2 to 10 nm resolution [1]. There remain challenges associated with imaging intact bacteria and pleomorphic viruses by cryo-ET. In particular, investigators cope with the low contrast of cryo-preserved, unstained spec...
متن کاملSingle-Particle Cryo-EM at Crystallographic Resolution
Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its limited resolution in the range of nanometer to subnanometer. Now, this method rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization o...
متن کاملUsing the Volta phase plate with defocus for cryo-EM single particle analysis
Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes th...
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ژورنال
عنوان ژورنال: Journal of Structural Biology
سال: 2011
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2011.03.020